cDNA Libraries and Expression Libraries MIT 7.01SC Fundamentals of Biology
ERIC LANDER: That worked. Let's do it again. Now, number 2. I would like to clone not the Arg1 gene, but let's say the beta globin gene from human. So let's take human beta globin. I want to clone it. So human beta globin, hemoglobin, it's a tetromer. It has four parts. It's got an alpha, an alpha, a beta, and a beta. Four proteins come together in a protein tetromer. And it's got two alphas, two betas. The beta subunit of hemoglobin is encoded by the human beta globin gene. That's the nomenclature here. All right. Let's clone beta globin. Same deal. How are we going to do it? Any takers? What should we start with? Yes? AUDIENCE: Find a restriction enzyme? ERIC LANDER: Find a restriction enzyme. I'll go to catalogue and try EcoRI today. OK? Now what? AUDIENCE: Add it to a bunch of [INAUDIBLE]. ERIC LANDER: So I'm going to start with human DNA? AUDIENCE: Yes. ERIC LANDER: Yes is a good answer. Yes. I'm going to start with human D...