Lec 27 MIT 7.014 Introductory Biology, Spring 2005
There was a little confusion with dideoxies in one sense, and some of these things like the PCR you're going to have to sort of sit down and actually think about it, but the principle of the dideoxies, if we were making a chain of beads that had a hook on one end and a little hole on the other, and we were joining these things together, we could make obviously, a chain that went on. And then we could hook another one in, and so on. And if we had a bunch of beads like this every now and then we threw in a very small number that didn't have the hook on the end, any time this particular chain were elongating and we put on one of these things, the chain would stop because you haven't got any where to join onto it. You added dideoxy into a polymerase reaction. A chain that gets the dideoxy doesn't have anything to join in the end, and that will stop. If we only added this, the entire reaction would stop, and everyone would come at the first time a dideoxy got i...