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Lecture 3 Measurement Techniques

ATUL BUTTE: So last week I talked about-- what did I talk about? I talked about an introduction to molecular biology for about 10 or 12 slides. And then we were going pretty quickly I covered a lot of this material on gene measurement techniques. And so we've already had one question, or one request, to talk about something that I actually flipped pretty quickly through, this concept of SAGE and how is SAGE used to measure microarrays. And actually it's a measure of RNA, and it's quite important that we actually talk about SAGE, I think. But I'm going to start the discussion with SAGE talking about microarrays again for a second. So I we talked about microarrays, how these are made grids of DNA. Each spot in the grid is looking for one particular RNA. And in fact, more than one spot might be actually hybridized into one RNA. It doesn't have to be one to one It could be many to one. And I just want to talk about the differences of the two technologies f...

Lecture 2 Introduction to Biology and Genomic Measurement

ATUL J. BUTTE: So I can and have in the past talked for about 6 hours on this subject. Today, we're just going to talk about the first of these, microbiology for the [? bioinformaticist. ?] And if we have time, then we can talk about gene measurement techniques, not just microarrays, but all sorts of different technologies depending on how fast or slow we go. So let's just get started then. So I have about 10 slides of basic biology, OK? If I see you start to yawn, then we'll just go faster. There's a lot that we can cover. So how many of you remember this kind of thing? Do you remember this? Do you remember this? Do you remember this? OK, so we can go pretty fast. So obviously, the key problem in biology or in all of life science is that organisms need to produce proteins for a variety of different things that they need to do over their lifetime. So the goal is proteins, but the organisms have to make the same proteins again and again in a very set patter...