TLC-Advanced MIT Digital Lab Techniques Manual
[MUSIC PLAYING] PROFESSOR: TLC Advanced. While the basic TLC video covers the essential steps for using thing layer chromatography, a few advanced techniques may also come in handy. For instance, learning how to cut your own glass TLC plates or pull your own spotters is essential in a research lab environment. It is also important to know how to visualize spots using TLC stains as well as UV light. This video will cover the advanced techniques involved in carrying out thin layer chromatography. In the basic TLC video, you learned how to set up a developing chamber, mark and spot a TLC plate, develop the plate, visualize the spots using a UV lamp, and calculate Rf values. You also learned how to choose a developing solvent and compare the identity of two compounds using a co-spot. This video will demonstrate some advanced techniques commonly used in research laboratories. You will learn how to cut glass TLC plates from large plates, how to pull spotters from capillary tube...