Buffers and pH Meter MIT Digital Lab Techniques Manual
[MUSIC PLAYING] PROFESSOR: Many chemistry and biochemistry procedures will require you to perform a reaction at a specific pH. To accomplish this task, you must understand how to both make a buffer and how to use a pH meter. This video will demonstrate two methods of buffer preparation as well as proper use of the pH meter. A buffer is an ionic compound that resists changes to its pH when strong acids or bases are added to it. The amount of strong acid or strong base that can be neutralized by a particular buffer is dictated by the buffer's capacity, which is determined by the concentration of weak acid and weak base in the buffer. A higher concentration of buffering reagents translates into greater buffering capacity. In the first part of this video, we will walk through two methods of buffer preparation. In the first example, you will need to prepare 1 liter of a 50 millimolar buffer for a protein that is stable at pH 7.3. The first step is choosing an appropriate b...