Lecture 3 Measurement Techniques
ATUL BUTTE: So last week I talked about-- what did I talk about? I talked about an introduction to molecular biology for about 10 or 12 slides. And then we were going pretty quickly I covered a lot of this material on gene measurement techniques. And so we've already had one question, or one request, to talk about something that I actually flipped pretty quickly through, this concept of SAGE and how is SAGE used to measure microarrays. And actually it's a measure of RNA, and it's quite important that we actually talk about SAGE, I think. But I'm going to start the discussion with SAGE talking about microarrays again for a second. So I we talked about microarrays, how these are made grids of DNA. Each spot in the grid is looking for one particular RNA. And in fact, more than one spot might be actually hybridized into one RNA. It doesn't have to be one to one It could be many to one. And I just want to talk about the differences of the two technologies f...